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產(chǎn)地 | 國(guó)產(chǎn)、進(jìn)口 |
品牌 | 上海滬崢 |
保存條件 | -20 °C |
貨號(hào) | HZK-9622 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
抗體名 | 玻璃粘連蛋白圄體,Anti-VTN/Vitronectin |
克隆性 | 是 |
靶點(diǎn) | 電詢 |
適應(yīng)物種 | 人、変鼠、小鼠、兔、雞、豬等 |
形態(tài) | 液體 |
宿主 | 兔、小鼠、羊 |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見說明書 |
濃度 | 99% |
免疫原 | 詳見說明 |
玻璃粘連蛋白抗體,Anti-VTN/Vitronectin抗體哪里有,上海滬崢廠家直銷,質(zhì)量穩(wěn)定、價(jià)格可靠,全國(guó)免費(fèi)運(yùn)輸。
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玻璃粘連蛋白抗體,Anti-VTN/Vitronectin抗體產(chǎn)品信息:
中文名稱:玻璃粘連蛋白抗體
英文名稱:Anti-VTN/Vitronectin
別名:Vitronectin; Complement S Protein; Epibolin; S Protein; Serum Spreading Factor; V75; Vitronectin V10 subunit; VN; VNT; VTN; S-protein; Serum-spreading factor; V75; Vitronectin V10 subunit; VTNC_HUMAN
抗體來源:Rabbit/Mouse
克隆類型:?jiǎn)慰?/span>/多抗
性 狀:Lyophilized or Liquid(凍干粉或液體)
濃 度:1mg/ml Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50%
亞 型:IgG
產(chǎn)品規(guī)格:0.1ml/0.2ml
交叉反應(yīng):人,大鼠,小鼠,犬,豬,羊等(Human, Mouse, Rat, Dog, Pig, Cow)
產(chǎn)品應(yīng)用:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蠟切片需做抗原修復(fù))not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user
分 子 量9/52kDa
研究領(lǐng)域心血管 免疫學(xué)
產(chǎn)品介紹background:
Vitronectin (also known as serum spreading factor, S protein of complement or epibolin) is one of the major multifunctional cell adhesive glycoproteins in mammalian plasma and serum. It is a monomeric acidic glycoprotein detected as a mixture of 75 kD and 65 kD polypeptides. Vitronectin binds to heparin, collagen, streptococci and variety of cultured cells. It also acts as an inhibitor of the complement cascade by binding to the C5b9 complex. Vitronectin promotes cell adhesion and spreading by binding through its cell attachment tripeptide Arg-Gly-Asp (RG-D), activity which is mediated by several different integrin receptors. Apart from the significance for identifying the molecule in the above situations, it also plays an important role in events such as embryonal development, deposition of vitronectin in a number of fibrotic disease states, carcinomas and metastases.
純化方式:親和層析純化
保存條件:-20℃,避免反復(fù)凍融
玻璃粘連蛋白抗體,Anti-VTN/Vitronectin抗體反應(yīng)特點(diǎn)
1.高度特異性,特異性是抗原抗體反應(yīng)的最主要特征,特定抗原決定簇只與特定抗體結(jié)合;
2.可逆性:抗原與抗體結(jié)合形成抗原抗體復(fù)合物的過程是一種動(dòng)態(tài)平衡, 其反應(yīng)式為:Ag+AbAg·Ab;
3.適當(dāng)濃度和比例;
4.敏感性:在測(cè)定血清中某一物質(zhì)的含量時(shí),化學(xué)比色法的敏感度為mg/ml水平,酶反應(yīng)測(cè)定法的敏感度約為5~10μg/ml,免疫測(cè)定中凝膠擴(kuò)散法的敏感度與酶反應(yīng)法相仿。
玻璃粘連蛋白抗體,Anti-VTN/Vitronectin抗體反應(yīng)的因素
關(guān)膜蛋白2抗體,Anti-VAMP21.電解質(zhì) :抗原抗體特異結(jié)合后,膠體粒便可發(fā)生脫水作用,此時(shí)易受電解質(zhì)影響,如有適當(dāng)濃度的電解質(zhì)存在就會(huì)使它們失去部分負(fù)電荷而相互凝聚,于是出現(xiàn)明顯的凝集或沉淀現(xiàn)象。
2.酸堿度:合適的pH為6-8。
3.溫度:37℃通常是抗原體反應(yīng)最適溫度。
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