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產(chǎn)地 | 國產(chǎn)、進口 |
品牌 | 上海滬崢 |
保存條件 | -20 °C |
貨號 | HZK-100109 |
應用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
抗體名 | TBR1蛋白圄體 |
克隆性 | 是 |
靶點 | 電詢 |
適應物種 | 人、変鼠、小鼠、兔、雞、豬等 |
形態(tài) | 液體 |
宿主 | 兔、小鼠、羊 |
亞型 | IgG |
標識物 | 詳見說明書 |
濃度 | 99% |
免疫原 | 詳見說明 |
TBR1蛋白抗體廠家直銷,上海滬崢質(zhì)量穩(wěn)定、價格可靠,全國免費運輸。
上海滬崢專業(yè)供應各類一抗、二抗、標記二抗、抗原等多種抗體產(chǎn)品,主要用于石蠟切片免疫組化,冰凍切片免疫組化,Elisa,WB,免疫熒光等科研實驗領域,抗體的相關標記:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,Alexa Fluor 647等,
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TBR1蛋白抗體產(chǎn)品信息:
中文名稱:TBR1蛋白抗體
英文名稱:Anti-TBR1
別名: A novel murine and human gene, TBR-1, encodes a putative transcription factor related to the Brachyrury (T) gene that is expressed only in postmitotic cells. T-brain-1 (TBR-1) mRNA is largely restricted to the cerebral cortex, where, during embryogenesis, it defines different regions that give rise to the palecortex, limbic cortex and neocortex (1-3). TBR-1, Pax-6 and Emx-1 are expressed in the mouse and chicken pallium. The pallio-subpallial boundary lies at the interface between the TBR-1 and Dlx-2 expression domains. Chicken genes homolgous to these mouse genes are expressed in topologically comparable patterns during development, suggesting that mouse and chicken may have similar histogenetic specification processes and field homologies (4). CASK/LIN-2, a membrane-associated guanylate kinase, is required for EGFR localization and signaling. In adult rat brain, CASK is concentrated at neuronal synapses and binds to the cell-surface proteins. CASK can interact with TBR-1, which is involved in forebrain development. CASK enters into the nucleus and binds to a specific DNA sequence (the T-element) in a complex with TBR-1. Thus, CASK acts as a coactivator of TBR-1 to induce transcription of T-element containing genes, including reelin (5); TBR1_HUMAN.
抗體來源:Rabbit/Mouse
克隆類型:單抗/多抗
性 狀:Lyophilized or Liquid(凍干粉或液體)
濃 度:1mg/ml Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50%
亞 型:IgG
產(chǎn)品規(guī)格:0.1ml/0.2ml
交叉反應:人,大鼠,小鼠,犬,豬,羊等(Human, Mouse, Rat, Dog, Pig, Cow)
產(chǎn)品應用:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蠟切片需做抗原修復)not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user
研究領域細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導
純化方式:親和層析純化
TBR1蛋白抗體注意事項:
1.抗體保存原則:避免反復凍融
2.短期保存的抗體:如抗體幾周內(nèi)就會使用,收到后推薦在2-8℃保存,2-8℃短暫保存數(shù)周對抗體活性沒有影響。
3.長期保存的抗體:如抗體需超過1個月后才會使用,請酌情分裝后于-20℃保存。
4.建議:將抗體保存在冰箱里層,避免溫度變化造成反復凍融。
*避免將抗體保存在自動除霜冰箱中。
反復凍融過程中,冰晶會對抗體的空間結構造成破壞,導致蛋白變性形成多聚體,從而降低抗體的結合能力,加快抗體降解速度。
TBR1蛋白抗體反應特點
1.高度特異性,特異性是抗原抗體反應的最主要特征,特定抗原決定簇只與特定抗體結合;
2.可逆性:抗原與抗體結合形成抗原抗體復合物的過程是一種動態(tài)平衡, 其反應式為:Ag+AbAg·Ab;
3.適當濃度和比例;
4.敏感性:在測定血清中某一物質(zhì)的含量時,化學比色法的敏感度為mg/ml水平,酶反應測定法
5.酸堿度:合適的pH為6-8。
6.溫度:37℃通常是抗原體反應最適溫度。
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